Project 1: Characterization of the interactions established by STAC3 in skeletal muscle
Skeletal muscle excitation-contraction (EC) coupling relies on the functional coupling of the voltage sensor (Cav1.1) and of the calcium release channel (RyR1). However, the precise mechanism of signal transmission during EC coupling between Cav1.1 and RyR1 remains elusive. Recently an adaptor protein, STAC3, was identified as essential for EC coupling. Ongoing work in our laboratory identified two interactions between STAC3 and Cav1.1 and an additional putative interaction between STAC3 and the RyR1. The goal of the PhD thesis project is to characterize the interactions STAC3 establishes within the EC coupling complex and their role in EC coupling.
Methods: Molecular biology, cell culture, primary zebrafish cultures, high- and super-resolution fluorescent microscopy, FRAP, patch-clamp and calcium imaging.
References: Campiglio et al. (2018) J Cell Physiol, under review; Campiglio et al. (2018) PNAS USA 115(6):1376-1381; Wong King Yuen et al. (2017) PNAS USA 114(45):E9520-E9528; Campiglio & Flucher (2017) Sci Rep. 7:41003.